Difference between revisions of "Part:BBa K1970002"
 
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This part is made up of a gene for red fluorescent protein (mCherry) controlled by the lambda promoter. A CI-repressor gene is attached to the parts ([https://parts.igem.org/Part:BBa_K608351]) , which represses expression of mCherry by binding to the lambda promoter.
 
This part is made up of a gene for red fluorescent protein (mCherry) controlled by the lambda promoter. A CI-repressor gene is attached to the parts ([https://parts.igem.org/Part:BBa_K608351]) , which represses expression of mCherry by binding to the lambda promoter.
  
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1970002 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1970000 parameters</partinfo>
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Latest revision as of 01:02, 28 October 2016


Red fluorescent protein controlled by CI-repressor

This part is made up of a gene for red fluorescent protein (mCherry) controlled by the lambda promoter. A CI-repressor gene is attached to the parts ([1]) , which represses expression of mCherry by binding to the lambda promoter.

Usage and Biology

The sensitivity of the CI repressor towards temperature in this biobrick was tested by growing overnight cultures inoculated with E. coli DH5alpha cells at 30 °C and 37 °C. After correcting for cell density in the cultures, the fluorescence of each culture was measured:

Excitation wavelength: 584 nm, emission wavelength: 620 nm

Average fluorescence at 30 °C was 3413

Average fluorescence at 37 °C was 13700

These results show that the CI repressor implemented in this part is effective at repressing gene expression when grown at 30 °C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1586
    Illegal AgeI site found at 1698
  • 1000
    COMPATIBLE WITH RFC[1000]