Difference between revisions of "Part:BBa K1970002"

Revision as of 01:00, 28 October 2016

Deoxynucleotide ribonuclease expressing plasmid which cuts off self inhibitory RBS tails

This part is made up of a gene for red fluorescent protein (mCherry) controlled by the lambda promoter. A CI-repressor gene is attached to the parts ([1]) , which represses expression of mCherry by binding to the lambda promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 341
Illegal NheI site found at 1575
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 619
Illegal XhoI site found at 1853
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1970000 short</partinfo>
-This part is made up of a gene for red fluorescent protein (RFP) controlled by the lambda promoter. A CI-repressor gene is attached to the parts, which represses expression of RFP by binding to the lambda promoter.
+This part is made up of a gene for red fluorescent protein (mCherry) controlled by the lambda promoter. A CI-repressor gene is attached to the parts ([https://parts.igem.org/Part:BBa_K608351]) , which represses expression of mCherry by binding to the lambda promoter.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+The sensitivity of the CI repressor towards temperature in this biobrick was tested by growing overnight cultures inoculated with E. coli DH5alpha cells at 30 °C and 37 °C. After correcting for cell density in the cultures, the fluorescence of each culture was measured:
+Excitation wavelength: 584 nm, emission wavelength: 620 nm
+Average fluorescence at 30 °C was 3413
+Average fluorescence at 37 °C was 13700
+These results show that the CI repressor implemented in this part is effective at repressing gene expression when grown at 30 °C.
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