Difference between revisions of "Part:BBa K2109110"

Revision as of 20:55, 27 October 2016

HDVR U1A-C75T

HDVR U1A-C75T is an inducible ribozyme that cleaves in the presence of 0.2 M imidazole. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR negative control U1A (Part:BBa_K2109109)is inducible in the presence of 0.2 M imidazole and also produces of transcript of ~250 bp when in vitro transcribed. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 58
Illegal NgoMIV site found at 87
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2109110 short</partinfo>
-HDVR U1A-C75T is an inducible ribozyme that cleaves in the presence of 0.2 M imidazole. It can be in vitro transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR negative control U1A (Part:BBa_K2109109)is inducible in the presence of  0.2 M imidazole and also produces of transcript of ~250 bp when in vitro transcribed. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.
+HDVR U1A-C75T is an inducible ribozyme that cleaves in the presence of 0.2 M imidazole. It can be <i>in vitro</i> transcribed via T7-RNA Polymerase to produce a transcript of approximately ~250 bp. The other HDVR negative control U1A (Part:BBa_K2109109)is inducible in the presence of  0.2 M imidazole and also produces of transcript of ~250 bp when in vitro transcribed. Ribozyme sequence obtained from (doi:10.1038) and was modified to conform to bio-brick standard.
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