Difference between revisions of "Part:BBa K1893031"

Revision as of 19:55, 27 October 2016

STAR target (pAD1.S5)

For designing a small transcriptional activating RNA through an anti-terminator mechanism, Chappell et. al. investigated transcriptional terminators from different plasmids and of different lengths and sequences. Of these, the sense pAD1 plasmid attenuator sequence no. 5 (pAD1.S5) and its associated STAR-antisense sequence no. 5 (pAD1.A5) pair gave the highest (94-fold) activation of the reporter gene, SFGFP [Chappell et al., 2015]. Thus we used the pAD1.S5 sequence as our STAR target-encoding DNA and the pAD1.A5 sequence as the STAR-antisense-encoding DNA.

When transcribed, this part produces the sense RNA which is also complementary to the STAR-antisense RNA. In the absence of STAR, it forms a hairpin structure and and prevent transcription of the downstream coding sequence.

Secondary Structure

File:Mfold-K1893031-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

@@ Line 8: / Line 8: @@
 | width=50% valign='top' style='border: 1px solid black'|
 <partinfo>BBa_K1893031 short</partinfo>
-* When transcribed, it produces the sense RNA which is also complementary to the STAR-antisense RNA. In the absence of STAR, it forms a hairpin structure and and prevent transcription of the downstream coding sequence.
+For designing a small transcriptional activating RNA through an anti-terminator mechanism, Chappell et. al. investigated transcriptional terminators from different plasmids and of different lengths and sequences. Of these, the sense pAD1 plasmid attenuator sequence no. 5 (pAD1.S5) and its associated STAR-antisense sequence no. 5 (pAD1.A5) pair gave the highest (94-fold) activation of the reporter gene, SFGFP [Chappell et al., 2015]. Thus we used the pAD1.S5 sequence as our STAR target-encoding DNA and the pAD1.A5 sequence as the STAR-antisense-encoding DNA.
+When transcribed, this part produces the sense RNA which is also complementary to the STAR-antisense RNA. In the absence of STAR, it forms a hairpin structure and and prevent transcription of the downstream coding sequence.
 |}