Difference between revisions of "Part:BBa K1976000:Experience"

 
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===User Reviews===
 
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We transposed our Biobrick onto a low copy vector (pSB4A5), because it is easier for plasmid curing afterwards. The sequencing results of the transformation into <i>E.&#8239;coli</i>&nbsp;JM109 showed in four cases four different DNA sequences. After a protein blast (using NCBI BLAST) we found a transposase between prefix and suffix of our vector. We assume, that the homologous region in the &lambda;-attP site is recognized by the transposase and it uses the similar sequences of prefix and suffix to translocate between these sites.
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        <img style="width: 95%; height: 140%; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/parts/c/ce/T--TU_Darmstadt--Blast_Transposase.png">
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            <b>Figure 1:</b> Blast of our sequencing results showing the ISAs1 family transposase (we expected BBa_K1976000 and got this) (NCBI blastx was used for this)
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Revision as of 16:32, 27 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1976000

User Reviews

UNIQ838e8ee16c9fb56f-partinfo-00000000-QINU

No review score entered. TSD

We transposed our Biobrick onto a low copy vector (pSB4A5), because it is easier for plasmid curing afterwards. The sequencing results of the transformation into E. coli JM109 showed in four cases four different DNA sequences. After a protein blast (using NCBI BLAST) we found a transposase between prefix and suffix of our vector. We assume, that the homologous region in the λ-attP site is recognized by the transposase and it uses the similar sequences of prefix and suffix to translocate between these sites.

Figure 1: Blast of our sequencing results showing the ISAs1 family transposase (we expected BBa_K1976000 and got this) (NCBI blastx was used for this)

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UNIQ838e8ee16c9fb56f-partinfo-00000003-QINU