Difference between revisions of "Part:BBa K1893012"

Latest revision as of 16:02, 27 October 2016

Constitutive STAR and anti-STAR (J23119+STAR+anti-STAR)

To regulate STAR activity, we designed an inhibitory RNA sequence (Anti-STAR). The Anti-STAR sequence is identical to a portion of the pAD1.S5 attenuator sequence, and is therefore also complementary to STAR. Anti-STAR has a higher affinity for STAR than the pAD1 attenuator does, so, when transcribed, Anti-STAR sequesters STAR and prevents it from interacting with the target sequence and activating transcription.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 154
Illegal NheI site found at 177
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 13:59, 27 October 2016 (view source) Cmoller (Talk \| contribs) ← Older edit		Latest revision as of 16:02, 27 October 2016 (view source) AkashB 10 (Talk \| contribs)
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	<partinfo>BBa_K1893012 short</partinfo>		<partinfo>BBa_K1893012 short</partinfo>

		+	To regulate STAR activity, we designed an inhibitory RNA sequence (Anti-STAR). The Anti-STAR sequence is identical to a portion of the pAD1.S5 attenuator sequence, and is therefore also complementary to STAR. Anti-STAR has a higher affinity for STAR than the pAD1 attenuator does, so, when transcribed, Anti-STAR sequesters STAR and prevents it from interacting with the target sequence and activating transcription.