Difference between revisions of "Part:BBa K1893018:Design"
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===Source=== | ===Source=== | ||
| − | LeuB was amplified from E. coli TURBO cells using PCR. Subsequent rounds of PCR were undertaken to add the B0035 ribosome binding site, and the biobrick prefix and suffix. This block was then ligated into BBa_K1893015 | + | LeuB was amplified from E. coli TURBO cells using PCR. Subsequent rounds of PCR were undertaken to add the B0035 ribosome binding site, and the biobrick prefix and suffix. This block was then ligated into [https://parts.igem.org/Part:BBa_K1893015 BBa_K1893015.] |
===References=== | ===References=== | ||
Latest revision as of 15:46, 27 October 2016
Arabinose inducible 3-isopropylmalate dehydrogenase (pBAD+leuB)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1098
Design Notes
The sequence for leuB was obtained from the NCBI, and no codon optimisation was required, as it is already part of the E. coli genome.
Source
LeuB was amplified from E. coli TURBO cells using PCR. Subsequent rounds of PCR were undertaken to add the B0035 ribosome binding site, and the biobrick prefix and suffix. This block was then ligated into BBa_K1893015.