Difference between revisions of "Part:BBa K2027013:Design"
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===Design Notes===
 
===Design Notes===
 
Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant
 
Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant
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[[File:AroG_Mut1_SV.png|500px|thumb|left|alt text]]
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[[File:AroG Mut1 SV|500px|thumb|left|alt text]]
  
  

Revision as of 15:02, 27 October 2016


Mutant Feedback-Resistant Version of AroG (Pro150Leu)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 934
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 241


Design Notes

Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant

File:AroG Mut1 SV
alt text


Source

https://parts.igem.org/Part:BBa_K1060000

References