Difference between revisions of "Part:BBa K1893013:Design"
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===Design Notes===
 
===Design Notes===
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This sequence was obtained through the use of inverse PCR on the 'j23119-STAR/pSB1C3' construct (BBa_K1893010) to remove the constitutive promoter j23119.  
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This sequence was obtained through the use of inverse PCR on the 'j23119-STAR/pSB1C3' construct [https://parts.igem.org/Part:BBa_K1893010 (BBa_K1893010)] to remove the constitutive promoter j23119.  
  
 
STAR Forward primer:
 
STAR Forward primer:

Latest revision as of 14:53, 27 October 2016


Small transcription activating RNA (STAR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was obtained through the use of inverse PCR on the 'j23119-STAR/pSB1C3' construct (BBa_K1893010) to remove the constitutive promoter j23119.

STAR Forward primer: TGAACTGTATACATTCCCCGC

Prefix Reverse primer: CTCTAGAAGCGGCCGCG

Source

The pAD1.A5 STAR sequence (STAR-antisense)

References

Chappell, J., Takahashi, M. & Lucks, J. (2015) Creating small transcription activating RNAs. Nature Chemical Biology. [Online] 11 (3), 214-220