Difference between revisions of "Part:BBa K1878006:Experience"

Revision as of 03:28, 27 October 2016

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Applications of BBa_K1878006

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UNIQ51af7f6fb6af6dad-partinfo-00000000-QINU UNIQ51af7f6fb6af6dad-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor in E. coli with a modified version of NarK (mNarK) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the results of our characterization indicate that transcription is downregulated</strong>.

Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.

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 <I>Rice iGEM 2016</I>
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-For the 2016 Team Rice project, we built a biosensor in <i>E. coli</i> with a modified version of NarK (mNarK) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the results of our characterization indicate that transcription is downregulated.
+For the 2016 Team Rice project, we built a biosensor in <i>E. coli</i> with a modified version of NarK (mNarK) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the <strong><em>results of our characterization indicate that transcription is downregulated</strong></em>.
 [[Image:MNarK_IRFP670_Hypoxia_Assay.jpg|thumb|left|800px|Fig 1. Results of part assay (construct output iRFP670) in aerobic, and anaerobic conditions -- oxygen was purged with nitrogen gas. Results are averaged over 8 samples that ran simultaneously in a single experiment.]]
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