Difference between revisions of "Part:BBa K1969002:Design"

(→‎Design Notes)
 
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===References===
 
===References===
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The original biobrick part is BBa_K209472, because the original sequence is not BioBrick compatible, we use site mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and we add a his-tag to its C terminus to minitor its membrane localization.

Latest revision as of 02:48, 27 October 2016


Homo sapiens adrenoceptor beta 2 (ADRB2) with 6 x His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562


Design Notes

We used site-specific mutagenesis (G at 192bp to A) to make it become assembly compatible.

Source

The sequence for Homo sapiens adrenoceptor beta 2 is from human genomic sequence, the sequence for 6 x his tag was added artificially.

References

The original biobrick part is BBa_K209472, because the original sequence is not BioBrick compatible, we use site mutation to eliminate the illegal PstI site by changing G at 192 bp to A, and we add a his-tag to its C terminus to minitor its membrane localization.