Difference between revisions of "Part:BBa K2043008:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Bpul-FBD1 is compatible with both pCOLA vector and with the PhytoBrick format. The sequence was checked and corrected for potential BpiI, BsaI and BsmBI recognition sites. These sites cannot be present in the coding sequence, because the BpiI, BsaI and BsmBI enzymes are widely used in phytobrick cloning (http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks). This BioBrick is the bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 1 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method. | |
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===Source=== | ===Source=== |
Revision as of 21:29, 26 October 2016
bpuI-FBD1 laccase codon optimized for E.coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 699
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Bpul-FBD1 is compatible with both pCOLA vector and with the PhytoBrick format. The sequence was checked and corrected for potential BpiI, BsaI and BsmBI recognition sites. These sites cannot be present in the coding sequence, because the BpiI, BsaI and BsmBI enzymes are widely used in phytobrick cloning (http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks). This BioBrick is the bpul gene (Bba_K2043007) codon optimized for E. coli was improved by addition of fabric binding domain 1 (FBD1) on the 3'-end of the sequence using Golden Gate assembly method.
Source
Bacilus pumillus