Difference between revisions of "Part:BBa K1976048"

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The Colicin E2 immunity protein (<a href="https://parts.igem.org/Part:BBa_K1976027">BBa_K1976027</a> and <a href="https://parts.igem.org/Part:BBa_K1976028">BBa_K1976028</a>) can be used in combination with the Colicin E2 DNase. Due to to the high binding affinity of the immunity protein to the DNase domain, it can be used to fuse proteins.
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The Colicin E2 immunity protein (<a href="https://parts.igem.org/Part:BBa_K1976027">BBa_K1976027</a> and <a href="https://parts.igem.org/Part:BBa_K1976028">BBa_K1976028</a>) can be used in combination with the Colicin E2 DNase. Due to to the high binding affinity of the immunity protein to the DNase domain (Kd ∼ 10− 15 M), it can be used to fuse proteins and in combination with a suppression mechanism for the immunity protein as a bacterial killswitch.
  
 
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Revision as of 19:09, 26 October 2016

Colicin E2 DNase domain "miniColicin"

Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name BBa_K1976027.

Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.


Usage

The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase. Due to to the high binding affinity of the immunity protein to the DNase domain (Kd ∼ 10− 15 M), it can be used to fuse proteins and in combination with a suppression mechanism for the immunity protein as a bacterial killswitch.

Characteristics


Molecular Weight 15.2 kDa
Residues 133
pI 9.06
[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php]
References

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]



[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007