Difference between revisions of "Part:BBa K1970000"
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This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes. Additionally the ribonucleases bind and inhibit the activity of each other to enable a XOR-gate like behaviour.
 
This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes. Additionally the ribonucleases bind and inhibit the activity of each other to enable a XOR-gate like behaviour.
  
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The two ribonucleases are under the controll of lacI and tetR promoter. Thus IPTG and tetracyline (or analogs thereof) can be used as logic gate inputs, while the mCherry fluorescence as output.
+
The two ribonucleases are under the control of lacI and tetR promoter. Thus IPTG and tetracyline (or analogs thereof) can be used as logic gate inputs, while the mCherry fluorescence as output.
  
 
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Revision as of 12:37, 26 October 2016


Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA.

This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes. Additionally the ribonucleases bind and inhibit the activity of each other to enable a XOR-gate like behaviour.

The two ribonucleases are under the control of lacI and tetR promoter. Thus IPTG and tetracyline (or analogs thereof) can be used as logic gate inputs, while the mCherry fluorescence as output.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 337
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 615
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]