Difference between revisions of "Part:BBa K1970000:Design"

 
 
Line 1: Line 1:
  
 
__NOTOC__
 
__NOTOC__
−
<partinfo>BBa_K1970004 short</partinfo>
+
<partinfo>BBa_K1970000 short</partinfo>
  
−
<partinfo>BBa_K1970004 SequenceAndFeatures</partinfo>
+
<partinfo>BBa_K1970000 SequenceAndFeatures</partinfo>
  
  
 
===Design Notes===
 
===Design Notes===
−
None in particular.
+
The sequences of the catalytic ssDNAs produced by the retrons in this biobrick were designed in order to maximize the ssDNAs' inhibitive effect on each other while keeping their affinities for their target cutting site the same. This was done using a combination of the ViennaRNA library and self-written scripts and programs that can be found here: https://github.com/TheAngryFox/retron_design.
  
  

Latest revision as of 12:34, 26 October 2016


Deoxynucleotide ribonuclease expressing plasmid which cuts off self inhibitory RBS tails


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 341
    Illegal NheI site found at 1575
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 619
    Illegal XhoI site found at 1853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences of the catalytic ssDNAs produced by the retrons in this biobrick were designed in order to maximize the ssDNAs' inhibitive effect on each other while keeping their affinities for their target cutting site the same. This was done using a combination of the ViennaRNA library and self-written scripts and programs that can be found here: https://github.com/TheAngryFox/retron_design.


Source

De novo DNA

References