Difference between revisions of "Part:BBa K1970000"

Revision as of 12:25, 26 October 2016

Single deoxynucleotide ribonuclease that binds to and degrades CI repressor mRNA.

This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes. Additionally the ribonucleases bind and inhibit the activity of each other to enable a XOR-gate like behaviour.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 337
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 615
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1970004 short</partinfo>
-This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes.
+This plasmid contains two genes that code for two separate deoxynucleotide ribonucleases. These are able to bind to the RBS end of a modified mCherry mRNA. The modified mCherry mRNA has a self inhibitory RBS tail, which prevents binding of the transcript to ribosomes. Cleaveage by one of the deoxynucleotide ribonucleases removes the self inhibitory tail from the RBS, freeing the transcript for translation by ribosomes. Additionally the ribonucleases bind and inhibit the activity of each other to enable a XOR-gate like behaviour.
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