Difference between revisions of "Part:BBa K1878000"

Revision as of 07:38, 26 October 2016

iRFP670

General Properties

iRFP670 is a fluorescent protein that has an excitation wavelength of 643 nm and an emission wavelength of 670 nm. It utilizes billiverdin as a chromophore. The extinction coefficient is 114,000 M^-1 cm^-1, and the quantum yield is 11.1%. It is twice as bright as iRFP713 (formerly known as iRFP). The photobleaching half-life is 290 s and pKa is 4.0. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.

The original citation is as follows: [citation]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 81
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 550
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 130
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 636
Illegal BsaI.rc site found at 193

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1878000 short</partinfo>
 <h3>General Properties</h3>
-<p>iRFP670 is a fluorescent protein that has an excitation wavelength of 643 nm and an emission wavelength of 670 nm. It utilizes billiverdin as a chromophore. The extinction coefficient is 114,000 M&#8722;1 cm&#8722;1, and the quantum yield is 11.1%. It is twice as bright as iRFP713 (formerly known as iRFP). The photobleaching half-life is 290 s and pKa is 4.0. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.</p>
+<p>iRFP670 is a fluorescent protein that has an excitation wavelength of 643 nm and an emission wavelength of 670 nm. It utilizes billiverdin as a chromophore. The extinction coefficient is 114,000 M<sup>-1</sup> cm<sup>-1</sup>, and the quantum yield is 11.1%. It is twice as bright as iRFP713 (formerly known as iRFP). The photobleaching half-life is 290 s and pKa is 4.0. Near-infrared fluorescent proteins are good for long-term in vivo imaging and exhibit high brightness in bacteria, mammalian cells, and mice.</p>
 <p>The original citation is as follows: [citation]</p>