Difference between revisions of "Part:BBa K1878006:Experience"

Revision as of 07:30, 26 October 2016

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Applications of BBa_K1878006

User Reviews

2016 Rice
UNIQ4fd70ddacd04bcec-partinfo-00000000-QINU UNIQ4fd70ddacd04bcec-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor in E. coli with a modified version of NarK (mNarK) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the results of our characterization indicate that transcription is downregulated.

Fig 1. Results of part assay in aerobic, and anaerobic, conditions

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 <I>Rice iGEM 2016</I>
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-For the 2016 Team Rice project, we built a biosensor in <i>E. coli</i> with a modified version of NarK (mNarK) and iRFP670 to fluoresce in the presence of nitric oxide. However, due to time constraints, we were unable to obtain characterization data for this part.
+For the 2016 Team Rice project, we built a biosensor in <i>E. coli</i> with a modified version of NarK (mNarK) and iRFP670 to fluoresce in anaerobic conditions. Despite literature description that NarK should be upregulated in anaerobic conditions, the results of our characterization indicate that transcription is downregulated.
+[[Image:MNarK_IRFP670_Hypoxia_Assay.jpg|thumb|left|800px|Fig 1. Results of part assay in aerobic, and anaerobic, conditions]]
 |}