Difference between revisions of "Part:BBa K1878009:Experience"
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<I>Rice iGEM 2016</I> | <I>Rice iGEM 2016</I> | ||
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| − | For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> | + | For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with SoxRS and iRFP713 to fluoresce in the presence of nitric oxide. Diethylenetriamine (DETA) was used a generator of nitric oxide (NO), since a challenge for the assay was from the fact that nitric oxide is a gas in standard conditions. |
[[Image:SoxRS_IRFP713_Nitric_Oxide_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay with NO induction from DETA.]] | [[Image:SoxRS_IRFP713_Nitric_Oxide_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay with NO induction from DETA.]] | ||
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Latest revision as of 07:04, 26 October 2016
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UNIQ531ae0f71b720832-partinfo-00000000-QINU UNIQ531ae0f71b720832-partinfo-00000001-QINU
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No review score entered. Rice iGEM 2016 |
For the 2016 Team Rice project, we built a biosensor construct in E. coli with SoxRS and iRFP713 to fluoresce in the presence of nitric oxide. Diethylenetriamine (DETA) was used a generator of nitric oxide (NO), since a challenge for the assay was from the fact that nitric oxide is a gas in standard conditions. |
