Difference between revisions of "Part:BBa K1878001:Experience"

 
Line 7: Line 7:
  
 
===User Reviews===
 
===User Reviews===
{|width='80%' style='border:1px solid gray'
 
|-
 
|width='10%'|
 
<partinfo>BBa_K1878001 AddReview number</partinfo>
 
<I>Rice iGEM 2016</I>
 
|width='60%' valign='top'|
 
For the 2016 Team Rice project, we used the fluorescent protein iRFP713 in <i>E. coli</i> for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
 
[[Image:IRFP713_Excitation_Spectrum.jpg|thumb|left|800px|Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 
[[Image:IRFP713_Emission_Spectrum.jpg|thumb|left|800px|Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 
|};
 
 
 
<!-- DON'T DELETE --><partinfo>BBa_K1878001 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1878001 StartReviews</partinfo>
 
<!-- Template for a user review  
 
<!-- Template for a user review  
Line 30: Line 19:
 
<!-- End of the user review template -->
 
<!-- End of the user review template -->
 
<!-- DON'T DELETE --><partinfo>BBa_K1878001 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1878001 EndReviews</partinfo>
 +
 +
{|width='80%' style='border:1px solid gray'
 +
|-
 +
|width='10%'|
 +
<partinfo>BBa_K1878001 AddReview number</partinfo>
 +
<I>Rice iGEM 2016</I>
 +
|width='60%' valign='top'|
 +
For the 2016 Team Rice project, we used the fluorescent protein iRFP713 in <i>E. coli</i> for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
 +
[[Image:IRFP713_Excitation_Spectrum.jpg|thumb|left|800px|Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 +
[[Image:IRFP713_Emission_Spectrum.jpg|thumb|left|800px|Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 +
|}

Latest revision as of 07:01, 26 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1878001

User Reviews

UNIQ599ba57b1c5d1dd3-partinfo-00000000-QINU UNIQ599ba57b1c5d1dd3-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we used the fluorescent protein iRFP713 in E. coli for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.

Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose
Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose