Difference between revisions of "Part:BBa R0010"
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==Usage in <i>Chromobacterium Violaceum</i>== | ==Usage in <i>Chromobacterium Violaceum</i>== | ||
| − | [http://2016.igem.org/Team:Tec-Monterrey Team Tec-Monterrey 2016] tested the Promoter BBa_R0010 in <i>C. Violaceum</i>. We were unable to confirm whether or not <i>C. Violaceum</i> had the lac operon, BLAST analysis of its genome suggested that it did not have this regulating system; our characterization demonstrates that the lac promoter can be used as a constitutive promoter in this chassis. There was no significant difference in the Fluorescence of the group of <i>C. Violaceum</i> with IPTG or without it, but in<i>E. Coli</i> it showed normal induction. Therefore we can confirm that this promoter is constitutive in <i>C. Violaceum</i>. | + | [http://2016.igem.org/Team:Tec-Monterrey Team Tec-Monterrey 2016] tested the Promoter BBa_R0010 in <i>C. Violaceum</i>. We were unable to confirm whether or not <i>C. Violaceum</i> had the lac operon, BLAST analysis of its genome suggested that it did not have this regulating system; our characterization demonstrates that the lac promoter can be used as a constitutive promoter in this chassis. There was no significant difference in the Fluorescence of the group of <i>C. Violaceum</i> with IPTG or without it, but in<i>E. Coli</i> it showed normal induction. Therefore we can confirm that this promoter is constitutive in <i>C. Violaceum</i>. https://static.igem.org/mediawiki/parts/4/4b/T--Tec-Monterrey--violaceumgrafiicauno.jpg https://static.igem.org/mediawiki/parts/7/76/T--Tec-Monterrey--violaceumgrafiicados.jpg |
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Revision as of 06:39, 26 October 2016
promoter (lacI regulated)
This part is an inverting regulator sensitive to LacI and CAP.
It contains two protein binding sites. The first binds the CAP protein, which is generally present in E.coli and is asocciated with cell health and availability of glucose. The second binds LacI protein.
- In the absence of LacI protein and CAP protein, this part promotes transcription.
- In the presence of LacI protein and CAP protein, this part inhibits transcription.
- LacI can be inhibited by [http://openwetware.org/wiki/IPTG IPTG].
- LacI is coded by BBa_C0010
Intrinsic noise value: 0.0707 (compare with R0011: 0.0040; R0051: 0.0869). See [http://2015.igem.org/Team:William_and_Mary William_and_Mary iGEM 2015]
Usage and Biology
This is a direct copy of bases 0365739 through 0365540 of the E. coli K-12 MG1655 genome, Genbank NC_000913 in reverse complement form. It is the natural promoter for the LacZYA operon. It includes the tail end of the LacI gene coding region, but no promoter region for that partial gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage in Chromobacterium Violaceum
[http://2016.igem.org/Team:Tec-Monterrey Team Tec-Monterrey 2016] tested the Promoter BBa_R0010 in C. Violaceum. We were unable to confirm whether or not C. Violaceum had the lac operon, BLAST analysis of its genome suggested that it did not have this regulating system; our characterization demonstrates that the lac promoter can be used as a constitutive promoter in this chassis. There was no significant difference in the Fluorescence of the group of C. Violaceum with IPTG or without it, but inE. Coli it showed normal induction. Therefore we can confirm that this promoter is constitutive in C. Violaceum. 
