Difference between revisions of "Part:BBa K1878001:Experience"

Revision as of 06:35, 26 October 2016

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Applications of BBa_K1878001

User Reviews

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we used the fluorescent protein iRFP713 for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.

Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose

Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose

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UNIQ2dae349f2bdd5750-partinfo-00000001-QINU UNIQ2dae349f2bdd5750-partinfo-00000002-QINU

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 <I>Rice iGEM 2016</I>
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-For the 2016 Team Rice project, we used the fluorescent protein iRFP670 for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
+For the 2016 Team Rice project, we used the fluorescent protein iRFP713 for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
 [[Image:IRFP713_Excitation_Spectrum.jpg|thumb|left|800px|Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 [[Image:IRFP713_Emission_Spectrum.jpg|thumb|left|800px|Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]