Difference between revisions of "Part:BBa K1878000:Experience"
Line 15: Line 15:
 
For the 2016 Team Rice project, we used the fluorescent protein iRFP670 for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
 
For the 2016 Team Rice project, we used the fluorescent protein iRFP670 for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.
 
[[Image:IRFP670_Excitation_Spectrum.jpeg|thumb|left|800px|Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 
[[Image:IRFP670_Excitation_Spectrum.jpeg|thumb|left|800px|Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
−
[[Image:IRFP670_Emission_Spectrum.jpg|thumb|left|800px|Fig 1. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
+
[[Image:IRFP670_Emission_Spectrum.jpg|thumb|left|800px|Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose]]
 
|};
 
|};
  

Revision as of 05:32, 26 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1878000

User Reviews

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we used the fluorescent protein iRFP670 for proof-of-concept experiments for our biosensors -- our future experiments would include genes encoding the enzymes to produce the small-molecule pigment Violacein, instead of fluorescent proteins. We placed the promoter pBAD upstream for protein production during these characterization experiments.

Fig 1. Results of part characterization for excitation wavelengths. Production was induced under promoter pBAD, with 2mM arabinose
Fig 2. Results of part characterization for emission wavelengths. Production was induced under promoter pBAD, with 2mM arabinose
;

UNIQ3916661955ba3d9a-partinfo-00000001-QINU UNIQ3916661955ba3d9a-partinfo-00000002-QINU