Difference between revisions of "Part:BBa K1954009:Design"
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The Arg box is the binding sequence (1) for ArgR, the common repressor of L-arginine biosynthetic genes for which the amino acid acts as a co-repressor. The ARG box is a binding sequence for ArgR and could be introduced into a high-copy number plasmid to titrate the repressor away from the regulatory sequences of biosynthetic genes.
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The Arg box is the binding sequence (1) for ArgR, the common repressor of L-arginine biosynthetic genes for which the amino acid acts as a co-repressor. This Biobrick could be introduced into a high-copy number plasmid to titrate the repressor away from the regulatory sequences of biosynthetic genes in order to enhance L-arginine biosynthesis.
  
 
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Revision as of 14:46, 25 October 2016


Arg box
The Arg box is the binding sequence (1) for ArgR, the common repressor of L-arginine biosynthetic genes for which the amino acid acts as a co-repressor. This Biobrick could be introduced into a high-copy number plasmid to titrate the repressor away from the regulatory sequences of biosynthetic genes in order to enhance L-arginine biosynthesis.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Tuchman M, Rajagopal BS, McCann MT, Malamy MH. Enhanced production of arginine and urea by genetically engineered Escherichia coli K-12 strains. Appl Environ Microbiol. 1997 Jan;63(1):33–8.