Difference between revisions of "Part:BBa K1972000"
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<partinfo>BBa_K1972000 parameters</partinfo>
 
<partinfo>BBa_K1972000 parameters</partinfo>
 
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===Figure 1：===
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===Figure 1. Bio-circuit after first optimization：===
 
[[File:T--SCUT-China_A--u0.png]]
 
[[File:T--SCUT-China_A--u0.png]]
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The native dszABC operon was rearranged and the promoter was replaced in order to avoid overlapping genes, increase the expression of the dsz genes, especially dszB, which encoded the rate-limiting enzyme of the 4S-pathway, and relieve inhibition. Besides, a synthetic dszD cassette which was not linked to the dszABC genes in engineered bacteria IGTS8 was also constructed (Figure 1).

Revision as of 14:20, 25 October 2016


dszA (a monooxygenase gene )

DszA is a monoxygenase which can convert DBTsulfone (DBTO2) to 2-(2-hydroxybiphenyl) sulfinate (HBPS) during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszA require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 775
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 64
    Illegal NgoMIV site found at 279
    Illegal NgoMIV site found at 583
    Illegal NgoMIV site found at 703
    Illegal AgeI site found at 238
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1087


Figure 1. Bio-circuit after first optimization：

T--SCUT-China A--u0.png

The native dszABC operon was rearranged and the promoter was replaced in order to avoid overlapping genes, increase the expression of the dsz genes, especially dszB, which encoded the rate-limiting enzyme of the 4S-pathway, and relieve inhibition. Besides, a synthetic dszD cassette which was not linked to the dszABC genes in engineered bacteria IGTS8 was also constructed (Figure 1).