Difference between revisions of "Part:BBa K1976048"
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<h1>Colicin E2 DNase domain "miniColicin"</h1> | <h1>Colicin E2 DNase domain "miniColicin"</h1> | ||
| − | Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name . | + | Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name <a href="https://parts.igem.org/Part:BBa_K1976027">BBa_K1976027</a>. |
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Revision as of 14:03, 25 October 2016
Colicin E2 DNase domain "miniColicin"
Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name BBa_K1976027.
Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.
Usage
The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase.
Characteristics
| Molecular Weight | 15.2 kDa |
| Residues | 133 |
| pI | 9.06 |
| [Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007