Difference between revisions of "Part:BBa K1954007"

Latest revision as of 13:36, 25 October 2016

Green Light Inducible bacteriocin Device (GLID)

We designed a ‘template’ green light-inducible device (GLID) with blue output (amilGFP), adapted from Synechocystis sp. PCC6803. There are three components of the system: the chromatic acclimation sensor (CcaS), the response regulator (CcaR) and the PcpcG2 promoter. CcaS is a histidine kinase which is activated by green light and can catalyze phosphorylation of CcaR, leading to its activation and induction of gene expression downstream of PcpcG2.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1829
Illegal NheI site found at 3697
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 3637
Illegal BamHI site found at 4125
Illegal XhoI site found at 3475
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2493
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 943
Illegal SapI site found at 3566

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 We designed a ‘template’ green light-inducible device (GLID) with blue output (amilGFP), adapted from <span style="font-style: italic;">Synechocystis</span> sp. PCC6803. There are three components of the system: the chromatic acclimation sensor (CcaS), the response regulator (CcaR) and the PcpcG2 promoter. CcaS is a histidine kinase which is activated by green light and can catalyze phosphorylation of CcaR, leading to its activation and induction of gene expression downstream of PcpcG2.
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