Difference between revisions of "Part:BBa K1954007:Design"
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In our design (Fig. 1), there are two independently inducible promoters (Ptac and pBAD) allowing for fine tuning of gene expression by modifying the amount of IPTG and arabinose. This device contains an improvement of the existing part of the device (BBa_K592019) because it constitutes a self-contained functional system with an improved RBS for PcpcG2 adapted from (16), suspected to increase the level of expression of the genes downstream of the promoter by about 40 times compared to the wild-type promoter.
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In our design (Fig. 1), there are two independently inducible promoters (Ptac and pBAD) allowing for fine tuning of gene expression by modifying the amount of IPTG and arabinose. This device contains an improvement of the existing part of the device (BBa_K592019) because it constitutes a self-contained functional system with an improved RBS for PcpcG2 adapted from (1), suspected to increase the level of expression of the genes downstream of the promoter by about 40 times compared to the wild-type promoter.
 
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<center> <img src = "https://static.igem.org/mediawiki/2016/8/8a/T--UCL--GLID.png" width="50%" height="50%"> </center>
 
<center> <img src = "https://static.igem.org/mediawiki/2016/8/8a/T--UCL--GLID.png" width="50%" height="50%"> </center>
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<p> <center> Fig. 1. Green light-inducible device concept diagram. </center> </p><br><br></html>
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<p> <center> Fig. 1. Green light-inducible device concept diagram. </center> </p><br><br>
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<li>Abe K, Miyake K, Nakamura M, Kojima K, Ferri S, Ikebukuro K, et al. Engineering of a green-light inducible gene expression system in S ynechocystis sp. PCC6803: Engineered green light gene expression system. Microb Biotechnol. 2014 Mar;7(2):177–83.</li>
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<partinfo>BBa_K1954007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1954007 SequenceAndFeatures</partinfo>

Revision as of 13:32, 25 October 2016


Green Light Inducible bacteriocin Device (GLID)
In our design (Fig. 1), there are two independently inducible promoters (Ptac and pBAD) allowing for fine tuning of gene expression by modifying the amount of IPTG and arabinose. This device contains an improvement of the existing part of the device (BBa_K592019) because it constitutes a self-contained functional system with an improved RBS for PcpcG2 adapted from (1), suspected to increase the level of expression of the genes downstream of the promoter by about 40 times compared to the wild-type promoter.

Fig. 1. Green light-inducible device concept diagram.



  • Abe K, Miyake K, Nakamura M, Kojima K, Ferri S, Ikebukuro K, et al. Engineering of a green-light inducible gene expression system in S ynechocystis sp. PCC6803: Engineered green light gene expression system. Microb Biotechnol. 2014 Mar;7(2):177–83.


    • Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      INCOMPATIBLE WITH RFC[12]
      Illegal NheI site found at 1829
      Illegal NheI site found at 3697
    • 21
      INCOMPATIBLE WITH RFC[21]
      Illegal BamHI site found at 3637
      Illegal BamHI site found at 4125
      Illegal XhoI site found at 3475
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal AgeI site found at 2493
    • 1000
      INCOMPATIBLE WITH RFC[1000]
      Illegal BsaI.rc site found at 943
      Illegal SapI site found at 3566


    Design Notes

    To be added


    Source

    To be added

    References