Difference between revisions of "Part:BBa K1976053"
(→Characteristics) |
|||
Line 83: | Line 83: | ||
</table> | </table> | ||
<br> | <br> | ||
+ | |||
+ | </html> | ||
+ | |||
+ | ===<h2>Characteristics</h2>=== | ||
+ | |||
+ | <h2>Sequence and Features</h2> | ||
+ | <partinfo>BBA_K1976053 SequenceAndFeatures</partinfo> | ||
+ | <br> | ||
+ | <html> | ||
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br> | [1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 11:24, 25 October 2016
Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters
![](https://static.igem.org/mediawiki/parts/8/8b/Immuno_colicine2.png)
Figure 1: The structure of the Im2 immunity protein and Colicin E2 in a complex.
Usage
This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to it.
Characteristics
Characteristics
Molecular Weight |
Colicin E2 Dnase domain
15.2 kDa Immunity Protein 10.0 kDa |
Residues | Colicin E2 Dnase domain 133 |
pI |
Colicin E2 Dnase domain
9.06 Immunity Protein 4.66 |
[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php] |
Characteristics
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 696
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 107
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007