Difference between revisions of "Part:BBa K1976053"

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                 <td>
 
                 <td>
 
                     Colicin E2 Dnase domain
 
                     Colicin E2 Dnase domain
−
                     15.2 kDa
+
                     <br>15.2 kDa<br>
 
                     Immunity Protein
 
                     Immunity Protein
−
                     10.0 kDa
+
                     <br>10.0 kDa<br>
 
                 </td>
 
                 </td>
 
             </tr>
 
             </tr>
Line 69: Line 69:
 
                 <td>
 
                 <td>
 
                     Colicin E2 Dnase domain
 
                     Colicin E2 Dnase domain
−
                     9.06
+
                     <br>9.06<br>
 
                     Immunity Protein
 
                     Immunity Protein
−
                     4.66
+
                     <br>4.66<br>
 
                 </td>
 
                 </td>
 
             </tr>
 
             </tr>

Revision as of 11:24, 25 October 2016

Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters



Figure 1: The structure of the Im2 immunity protein and Colicin E2 in a complex.


Usage

This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to it.

Characteristics

Characteristics


Molecular Weight Colicin E2 Dnase domain
15.2 kDa
Immunity Protein
10.0 kDa
Residues Colicin E2 Dnase domain 133
pI Colicin E2 Dnase domain
9.06
Immunity Protein
4.66
[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php]

[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007