Difference between revisions of "Part:BBa K1976048"
Line 75: | Line 75: | ||
</table> | </table> | ||
<b>References</b> | <b>References</b> | ||
− | + | </html> | |
<h2>Sequence and Features</h2> | <h2>Sequence and Features</h2> | ||
<partinfo>BBA_K1976048 SequenceAndFeatures</partinfo> | <partinfo>BBA_K1976048 SequenceAndFeatures</partinfo> | ||
<br> | <br> | ||
+ | |||
<br> | <br> | ||
+ | <html> | ||
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br> | [1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br> | ||
</body> | </body> | ||
</html> | </html> |
Revision as of 11:12, 25 October 2016
Colicin E2 DNase domain "miniColicin"
Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.
Usage
The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase.
Characteristics
Molecular Weight | 15.2 kDa |
Residues | 133 |
pI | 9.06 |
[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php] |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 28
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007