Difference between revisions of "Part:BBa K2027035"

 
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<partinfo>BBa_K2027035 short</partinfo>
 
<partinfo>BBa_K2027035 short</partinfo>
  
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LeorOrange-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein
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This part was created using a synthetically produced chromoprotein from the DNA2.0 chromoprotein paintbox [1] and adding a cellulose binding domain from the iGEM registry (<partinfo>BBa_K1321366</partinfo>) and a FLAG, lumino and 6x histidine tag.
  
  
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<partinfo>BBa_K2027035 parameters</partinfo>
 
<partinfo>BBa_K2027035 parameters</partinfo>
 
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==References==
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[1] https://www.dna20.com/products/protein-paintbox#3

Revision as of 08:59, 25 October 2016


LeorOrange-Cellulose Binding Domain-Flag-Lumio-His-tag Fusion Protein

This part was created using a synthetically produced chromoprotein from the DNA2.0 chromoprotein paintbox [1] and adding a cellulose binding domain from the iGEM registry (BBa_K1321366) and a FLAG, lumino and 6x histidine tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 52
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 464
    Illegal NgoMIV site found at 587
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 743


References

[1] https://www.dna20.com/products/protein-paintbox#3