Difference between revisions of "Part:BBa K1916100"

Revision as of 06:35, 25 October 2016

Ara-Inducible NpF2164g5 Expression System

A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain fused to the C-terminus for purification. Expression regulated by a pBAD promoter. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and cells appear vibrant blue-green under normal light.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 131
Illegal SpeI site found at 686
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
Illegal SpeI site found at 686
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 131
Illegal SpeI site found at 686
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 131
Illegal SpeI site found at 686
Illegal NgoMIV site found at 1182
Illegal AgeI site found at 1272
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1916100 short</partinfo>
-The NpF2164g5 protein (see BBa_K1916000) under control of a strong pBAD promoter.
+A GAF domain of the cyanobacteriochrome (CBCR) NpF2164 with an intein/chitin-binding domain fused to the C-terminus for purification. Expression regulated by a pBAD promoter. As with most CBCRs, NpF requires a phycocyanobilin co-factor, and must therefore be expressed in a system with heme oxygenase (BBa_I15008) and PcyA (BBa_I15009). NpF2164g5 expresses well in E. coli and cells appear vibrant blue-green under normal light.
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