Difference between revisions of "Part:BBa K2130013"

 
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Example(PAM in brackets):
 
Example(PAM in brackets):
 
5'-NNNN...NNN(NGG)-3'
 
5'-NNNN...NNN(NGG)-3'
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Evaluation studies from our team have compared the editing efficiency of this part compared to other Cas9/Cpf1.
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<img src="https://static.igem.org/mediawiki/2016/f/fc/T--NTU-Singapore--bigtest.jpg" height="60%"width="75%"></center>
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<img src="https://static.igem.org/mediawiki/2016/e/e2/T--NTU-Singapore--CDStest.jpg" height="60%"width="75%"></center>
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<img src=" https://static.igem.org/mediawiki/2016/b/bb/T--NTU-Singapore--seed.jpg" height="60%"width="75%"></center>
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Revision as of 17:35, 24 October 2016


SpCas9

This past is a human condon optimised SpCas9. SpCas9 recognises an 5'-NGG-3' PAM at the 3' end of the target sequence.

Example(PAM in brackets): 5'-NNNN...NNN(NGG)-3'

Evaluation studies from our team have compared the editing efficiency of this part compared to other Cas9/Cpf1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 341
    Illegal BglII site found at 1136
    Illegal BamHI site found at 1430
    Illegal XhoI site found at 1936
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1168
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 259
    Illegal BsaI.rc site found at 1501
    Illegal BsaI.rc site found at 2857
    Illegal BsaI.rc site found at 3280
    Illegal BsaI.rc site found at 3292
    Illegal BsaI.rc site found at 4159
    Illegal SapI.rc site found at 2964
    Illegal SapI.rc site found at 3546
    Illegal SapI.rc site found at 3561