Difference between revisions of "Part:BBa K2130007"

Latest revision as of 13:56, 24 October 2016

AsCpf1

Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)

Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 25
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 407
Illegal BglII site found at 1949
Illegal BglII site found at 2258
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3493
Illegal NgoMIV site found at 3954
Illegal NgoMIV site found at 3973
1000
COMPATIBLE WITH RFC[1000]

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 Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)
 Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.
+<html><center><img src="https://static.igem.org/mediawiki/parts/b/be/AsCpf1.PNG" height="60%"width="75%">
+<img src="https://static.igem.org/mediawiki/2016/f/fc/T--NTU-Singapore--bigtest.jpg" height="60%"width="75%"></center>
+<img src="https://static.igem.org/mediawiki/2016/e/e2/T--NTU-Singapore--CDStest.jpg" height="60%"width="75%"></center>
+<img src=" https://static.igem.org/mediawiki/2016/b/bb/T--NTU-Singapore--seed.jpg" height="60%"width="75%"></center>
+</html>
 <!-- Add more about the biology of this part here