Difference between revisions of "Part:BBa K2130007"
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Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1) | Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1) | ||
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+ | Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease. | ||
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Revision as of 13:45, 24 October 2016
AsCpf1
Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)
Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 25
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 407
Illegal BglII site found at 1949
Illegal BglII site found at 2258 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 3493
Illegal NgoMIV site found at 3954
Illegal NgoMIV site found at 3973 - 1000COMPATIBLE WITH RFC[1000]