Difference between revisions of "Part:BBa K2130007"

 
Line 4: Line 4:
  
 
Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)
 
Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)
 +
 +
Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease. 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 13:45, 24 October 2016


AsCpf1

Human codon optimised Acidaminococcus sp. BV3L6 (AsCpf1)

Similar to LbCpf1, it depends on TTTN PAM for target recognition and cleavage. The optimal gRNA length is 19-23 nucleotides. Cpf1 is proven to achieve more specific cleavage activity compared to Cas9 endonclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 25
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 407
    Illegal BglII site found at 1949
    Illegal BglII site found at 2258
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3493
    Illegal NgoMIV site found at 3954
    Illegal NgoMIV site found at 3973
  • 1000
    COMPATIBLE WITH RFC[1000]