Difference between revisions of "Part:BBa K892008:Experience"
| Line 16: | Line 16: | ||
|width='60%' valign='top'| | |width='60%' valign='top'| | ||
| − | This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter <partinfo>BBa_K914003</partinfo>, a strong RBS <partinfo>BBa_B0030</partinfo>) and OsmY amplified with a C-terminal HA tag. The device showed secretion of OsmY under rhamnose-regulated conditions. | + | This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter <partinfo>BBa_K914003</partinfo>, a strong RBS <partinfo>BBa_B0030</partinfo>) and OsmY amplified with a C-terminal HA tag resulting in the following composite part <partinfo>BBa_K1963007</partinfo>. The device showed secretion of OsmY under rhamnose-regulated conditions. |
We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli <partinfo>BBa_K1963005</partinfo> and Serratia <partinfo>BBa_K1963006</partinfo>). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the <i>rhamnose</i> promoter in order test for rhamnose-regulated expression of an sRNA secretion system | We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli <partinfo>BBa_K1963005</partinfo> and Serratia <partinfo>BBa_K1963006</partinfo>). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the <i>rhamnose</i> promoter in order test for rhamnose-regulated expression of an sRNA secretion system | ||
Latest revision as of 12:24, 24 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K892008
User Reviews
UNIQ5d3c422aaedbb72d-partinfo-00000000-QINU
|
•••••
iGEM Dundee 2016 |
This biobrick part was used to assemble a device that contains a rhamnose-responsive promoter BBa_K914003, a strong RBS BBa_B0030) and OsmY amplified with a C-terminal HA tag resulting in the following composite part BBa_K1963007. The device showed secretion of OsmY under rhamnose-regulated conditions. We also used this OsmY biobrick to create two translational fusion proteins (Hfq from E. coli BBa_K1963005 and Serratia BBa_K1963006). These two biobricks were then amplified with a C-terminal HA tag and hooked up to the rhamnose promoter in order test for rhamnose-regulated expression of an sRNA secretion system |
User Reviews
UNIQ5d3c422aaedbb72d-partinfo-00000007-QINU UNIQ5d3c422aaedbb72d-partinfo-00000008-QINU