Difference between revisions of "Part:BBa K2043005:Design"

 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Miau
+
His-tag added for enhanced purification. <br>
 +
Gene was codon optimised for expression in <i>E. coli</i>
  
  

Revision as of 12:08, 24 October 2016


xylE-FBD1 from Pseudomonas putida codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 700
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag added for enhanced purification.
Gene was codon optimised for expression in E. coli


Source

Pseudomonas putida

References