Difference between revisions of "Part:BBa K2043001:Design"
Line 9: Line 9:
 
His-tag is added in the C-terminal for optimised protein purification
 
His-tag is added in the C-terminal for optimised protein purification
 
<i>catA</i> was codon optimised for <i>E. coli</i>.
 
<i>catA</i> was codon optimised for <i>E. coli</i>.
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===Source===
 
===Source===
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===References===
 
===References===
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Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433. <br><br>
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 +
NCBI Reference Sequence: YP_004995593.1

Revision as of 11:33, 24 October 2016


catA from Acinetobacter pittii, codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag is added in the C-terminal for optimised protein purification catA was codon optimised for E. coli.

Source

Acinetobacter pittii's genome

References

Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433.

NCBI Reference Sequence: YP_004995593.1