Difference between revisions of "Part:BBa K2027037:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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TBA
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Below is the forward and reverse sequencing verification for the part, showing that the digestion left the heterotrimeric coiled coil and bacterial collagen domain intact.
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https://static.igem.org/mediawiki/parts/4/41/T--Stanford-Brown--s2seqver.png
  
 
===Source===
 
===Source===

Revision as of 09:16, 24 October 2016


Bacterial Collagen with Coiled-Coil Heterotrimerization Domain 2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 75
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 72
    Illegal BsaI.rc site found at 65


Design Notes

Below is the forward and reverse sequencing verification for the part, showing that the digestion left the heterotrimeric coiled coil and bacterial collagen domain intact.

T--Stanford-Brown--s2seqver.png

Source

TBA

References