Difference between revisions of "Part:BBa K2027002"
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<partinfo>BBa_K2027002 short</partinfo>
 
<partinfo>BBa_K2027002 short</partinfo>
  
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This part results from golden gate assembly via BsaI digest of [[BBa_K2027004]], [[BBa_K2027005]], and [[BBa_K2027006]]. Some restriction digest difficulties mean that this construct does not yet physically exist, but read more about it on the design page and the wiki[http://2016.igem.org/Team:Stanford-Brown/SB16_BioMembrane_Collagen].
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This part results from golden gate assembly via BsaI digest of [[BBa_K2027004]], [[BBa_K2027005]], and [[BBa_K2027006]]. Some restriction digest difficulties mean that this construct does not yet physically exist, but read more about it on the wiki[http://2016.igem.org/Team:Stanford-Brown/SB16_BioMembrane_Collagen].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:01, 24 October 2016


Long Bacterial Collagen Fiber Monomer with Cross-linking Domains

This part results from golden gate assembly via BsaI digest of BBa_K2027004, BBa_K2027005, and BBa_K2027006. Some restriction digest difficulties mean that this construct does not yet physically exist, but read more about it on the wiki[http://2016.igem.org/Team:Stanford-Brown/SB16_BioMembrane_Collagen].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 175
    Illegal SapI site found at 889
    Illegal SapI site found at 1612
    Illegal SapI.rc site found at 70
    Illegal SapI.rc site found at 784
    Illegal SapI.rc site found at 1507