Difference between revisions of "Part:BBa K1976054"
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         <h1>Colicin E2 DNase with B0034 and T7 promoter</h1>
 
         <h1>Colicin E2 DNase with B0034 and T7 promoter</h1>
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Generator for the Colicin DNase domain <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a>.
 
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Revision as of 18:13, 23 October 2016

Colicin E2 DNase with B0034 and T7 promoter

Generator for the Colicin DNase domain BBa_K1976048.

Figure 1:Figure 5: growth of BL21 E. coli transformed with a): BBa_K1976054 and b): BBa_K1976055.


Usage

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007