Difference between revisions of "Part:BBa K1976053"
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The Colicin E2 immunity protein (<a href="https://parts.igem.org/Part:BBa_K1976027">BBa_K1976027</a> and <a href="https://parts.igem.org/Part:BBa_K1976028">BBa_K1976028</a>) can be used in combination with the Colicin E2 DNase domain, or this part as an inhibitor. Since it binds very tightly to the immunity protein, it can also be used in fusing proteins to one another.
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This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to it.
  
 
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Revision as of 17:58, 23 October 2016

Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters



Figure 1: The structure of the Im2 immunity protein and Colicin E2 in a complex.


Usage

This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to it.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007