Difference between revisions of "Part:BBa K1976054"
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<b>Figure 1:</b>Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation. <b>Note:</b> this is not actually this part but <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a>, a 5 amino acids shorter version
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<b>Figure 1:</b>Figure 5: growth of BL21 E. coli transformed with a): BBa_K1976054 and b): BBa_K1976055.  
 
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Revision as of 17:19, 23 October 2016

Colicin E2 DNase with B0034 and T7 promoter

Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name.
This part can be created by digesting Colicin E2 with Trypsin.

Figure 1:Figure 5: growth of BL21 E. coli transformed with a): BBa_K1976054 and b): BBa_K1976055.


Usage

The Colicin E2 immunity protein (BBa_K1976027 and BBa_K1976028) can be used in combination with the Colicin E2 DNase domain, or this part as an inhibitor. Since it binds very tightly to the immunity protein, it can also be used in fusing proteins to one another.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 46
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007