Difference between revisions of "Part:BBa K1976048"
Line 10: Line 10:
 
     <body>
 
     <body>
 
         <h1>Colicin E2 immunity protein</h1>
 
         <h1>Colicin E2 immunity protein</h1>
−
This part encodes for the wild-type immunity protein inhibiting the nicking DNase Colicin E2. According to molecular dynamics simulations this protein inhibits also miniColicin (<a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a> and <a href="https://parts.igem.org/Part:BBa_K1976049">BBa_K1976049</a>).
+
Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .
 
<br>
 
<br>
 
<br>
 
<br>

Revision as of 16:36, 23 October 2016

Colicin E2 immunity protein

Colicin E2 is a peptide, that is toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name .

Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.


Usage

The Colicin E2 immunity protein can be used in combination with the Colicin E2 DNase or its engineered truncated variant miniColicin (BBa_K1976048 and BBa_K1976049).

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007