Difference between revisions of "Part:BBa K2180009"
| Line 3: | Line 3: | ||
<partinfo>BBa_K2180009 short</partinfo> | <partinfo>BBa_K2180009 short</partinfo> | ||
| − | + | The sequence is based on a previous IGEM part made by the group Antiquity (2003-01-31) coding for LacI + LVA for E. coli: | |
| + | <br>https://parts.igem.org/Part:BBa_C0012 | ||
| + | <br>This biobrick is the coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive. | ||
| + | <br>We had to improve the codon usage for B. subtilis | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 15:39, 23 October 2016
LacI-LVA for B. subtilis
The sequence is based on a previous IGEM part made by the group Antiquity (2003-01-31) coding for LacI + LVA for E. coli:
https://parts.igem.org/Part:BBa_C0012
This biobrick is the coding sequence of LacI protein + LVA (rapid degradation tail) wich improve the switch time and make it more sensitive.
We had to improve the codon usage for B. subtilis
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 178
Illegal AgeI site found at 966 - 1000COMPATIBLE WITH RFC[1000]