Difference between revisions of "Part:BBa K2180006"

Latest revision as of 15:33, 23 October 2016

PLacO - Promoter LacO for B. subtilis

The part is made of a previous IGEM part made by the team iGEM12_LMU-Munich (2012-07-16) :
https://parts.igem.org/Part:BBa_K823002
We add two LacI binding sites (Gilbert and Maxam, PNAS, 1973)
This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI operator binding sites and a SacII restriction site after them. They allow us to replace the LacI binding sites by other operating sequences. A SalI restriction sites is presents before the PlepA sequence and a NdeI restriction site after the RBS that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2180006 short</partinfo>
-This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence and a NdeI restriction site after the RBS that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.
+The part is made of a previous IGEM part made by the team iGEM12_LMU-Munich   (2012-07-16) :
+<br>https://parts.igem.org/Part:BBa_K823002
+<br>We add two LacI binding sites (Gilbert and Maxam, PNAS, 1973)
+<br>This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI operator binding sites and a SacII restriction site after them. They allow us to replace the LacI binding sites by other operating sequences. A SalI restriction sites is presents before the PlepA sequence and a NdeI restriction site after the RBS that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.
 <!-- Add more about the biology of this part here