Difference between revisions of "Part:BBa K2180004"
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This is the assembly of our basic parts BBa_K2180009 , BBa_K2180007 and BBa_K2180008. | This is the assembly of our basic parts BBa_K2180009 , BBa_K2180007 and BBa_K2180008. | ||
<br>The NcoI restriction site is overlapping parts BBa_K2180009 and BBa_K2180007 at the Start codon of LacI + LVA. | <br>The NcoI restriction site is overlapping parts BBa_K2180009 and BBa_K2180007 at the Start codon of LacI + LVA. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 15:29, 23 October 2016
Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator
This is the assembly of our basic parts BBa_K2180009 , BBa_K2180007 and BBa_K2180008.
The NcoI restriction site is overlapping parts BBa_K2180009 and BBa_K2180007 at the Start codon of LacI + LVA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1360
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 377
Illegal AgeI site found at 1165 - 1000COMPATIBLE WITH RFC[1000]