Difference between revisions of "Part:BBa K2180003"

 
 
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<partinfo>BBa_K2180003 short</partinfo>
 
<partinfo>BBa_K2180003 short</partinfo>
  
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We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. The regulatory region is composed by two DegU binding (one forward, one reverse) sites wich are derepressed in the presence of sucrose.
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The sequence of the regulatory region comes from this site
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<br>We added two restriction sites SacII before and PacI after the regulatory region in order to use it in our project.
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<br>They allow us to replace a regulatory region with another regulatory region.
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<br>The regulatory region is composed by two DegU binding (one forward, one reverse) sites which are derepressed in the presence of sucrose.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:28, 23 October 2016


Sucrose Operator - DegU Binding Site

The sequence of the regulatory region comes from this site
We added two restriction sites SacII before and PacI after the regulatory region in order to use it in our project.
They allow us to replace a regulatory region with another regulatory region.
The regulatory region is composed by two DegU binding (one forward, one reverse) sites which are derepressed in the presence of sucrose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]