Difference between revisions of "Part:BBa K1893017"
| Line 9: | Line 9: | ||
We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded fluorescence in a BMG plate reader. | We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded fluorescence in a BMG plate reader. | ||
| + | |||
| + | [[File:AraC_TOP10.png|700px|center|]] | ||
| + | |||
| + | [[File:Transfer_curve_arac.png|700px|center|]] | ||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K1893017 SequenceAndFeatures</partinfo> | <partinfo>BBa_K1893017 SequenceAndFeatures</partinfo> | ||
Revision as of 15:16, 23 October 2016
Arabinose inducible GFP (pBAD+GFP)
A GFP reporter under control of a pBAD promoter, used to characterise the activation range of the pBAD promoter
Usage and Biology
Without any arabinose in the cell, araC forms a homodimer, binding to operator sites upstream of the pBAD promoter, generating an inhibitory loop in the DNA that prevents access by the RNAP holoenzyme. In the absence of glucose, and the presence of L-arabinose, the dimer dissociates from the DNA, allowing transcription of GFP.
We added varying concentrations of arabinose (optimum concentration ranges were obtained from (sloveniaon igem characterisation), and recorded fluorescence in a BMG plate reader.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2017
Illegal SapI site found at 1098